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Table 2 Relative abundance of B.subtilis, B. cereus and Lys. fusiformis in larvae, pupae and sand flies reared in PBS-treated chow (control) or chow spiked with B. subtilis

From: The paratransgenic sand fly: A platform for control of Leishmania transmission

 

PBS-treated chow

B. subtilis spiked chow

 

B. subtilis

B. cereus

Lys fusiformis

B. subtilis

B. cereus

Lys fusiformis

Larvae

2.4%

20%

75%

99%

0.9%

 

Pupae

 

22%

70%

94%

2.1%

 

Sand flies

0.6%

32%

67%

74%

25%

1%

  1. The relative abundance of each bacterium was defined as the percentage of the total CFU of each bacterium divided by the total CFU per developmental stage. B. cereus and Lys fusiformis persisted through pupation with little to no loss in relative abundance in insects that were allowed to develop in PBS-treated chow. Although both organisms are displaced by the exogenous B. subtilis, they were still present in the emergent sand flies. Exogenous B. subtilis was able to displace most endogenous bacteria in the larval and pupal stages of growth.