Figure 5From: High quality RNA isolation from Aedes aegypti midguts using laser microdissection microscopyAmplification of target mRNA by real-time PCR (RT-PCR) assays. Total RNA isolated from laser microdissected tissues of Carnoy's-fixed (red lines), Bouin's (blue), or fresh snap-frozen (black lines) midguts of Aedes aegypti was used as templates in the RT-PCR assays in duplicate. The above are representative results of the duplicate assay.Back to article page