Table 1 Primer sequences used to amplify the target transcripts from total RNAs isolated from LMM-cut Aedes aegypti midgut tissues.
From: High quality RNA isolation from Aedes aegypti midguts using laser microdissection microscopy
Gene ID | Primer sequence (5' to 3') | Product size | Name |
|---|---|---|---|
DQ440299 | Forward: AACCAAGCAAACCCAAACC Reverse: AGGATACCGTTGGCATCG | 195 bp | Heat shock cognate 70 |
AAEL015246 and AAEL012410 | Forward:GCGGACGTAACTCATCCAC Reverse: CCCTGGTGACTGCAGAGATAG | 498 bp | AGO1a and b |
AAEL017251 | Forward: TCAAGCAGACGAACCAAA Reverse: ATGGCACGAAGTTCTATGG | 103 bp | AGO2 |
AAEL001466 | Forward: TCTAACGCATACGCACCAC Reverse: TGTCGTCACCAGAGCAATG | 130 bp | hypothetical protein |