Skip to main content

Advertisement

Table 2 Yields of total RNAs prepared from microdissected Aedes aegypti midgut tissues.

From: High quality RNA isolation from Aedes aegypti midguts using laser microdissection microscopy

   24 Hours Post Bloodmeal 48 Hours Post Bloodmeal
Sample   Fresh frozen Bouin's Carnoy's Fresh frozen Bouin's Carnoy's
RNA Conc. (pg/μl) 1* 883 806 244 952 515 659
  2 392 331 413 127 14 100
Total Yield (ng/20 μl) 1* 17.7 16.1 4.9 19.0 10.3 13.2
  2 7.8 6.6 8.3 2.5 0.3 2.0
  1. Two independent preparations were performed. The samples denoted by an asterisk (*) are the ones, of which electrophoretic image are shown in Figure 4.