Table 3 Threshold cycle (Ct) values of quantitative real-time PCR (qRT-PCR) amplification.
From: High quality RNA isolation from Aedes aegypti midguts using laser microdissection microscopy
24 hour | 48 hour | ||||
|---|---|---|---|---|---|
Gene | Fixative | 1 st | 2 nd | 1 st | 2 nd |
Fresh | 16.44 | 22.97 | 16.75 | 23.75 | |
hsc70 | Bouin's | 19.50 | 32.45 | 22.49 | 27.44 |
Carnoy's | 16.98 | 16.34 | 16.59 | 17.20 | |
Fresh | 27.32 | 34.87 | 26.22 | 34.94 | |
ago1a & b | Bouin's | 30.50 | 37.77 | 33.61 | 37.91 |
Carnoy's | 26.81 | 27.26 | 25.43 | 28.63 | |
Fresh | 23.17 | 27.57 | 22.34 | 27.79 | |
ago2 | Bouin's | 24.77 | 30.35 | 27.04 | 28.36 |
Carnoy's | 22.84 | 23.90 | 23.11 | 23.58 | |
Fresh | 24.78 | 28.07 | 24.93 | 28.62 | |
hypo. protein | Bouin's | 25.66 | 33.36 | 31.70 | 29.27 |
Carnoy's | 24.43 | 24.94 | 26.00 | 27.03 | |