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Table 3 Threshold cycle (Ct) values of quantitative real-time PCR (qRT-PCR) amplification.

From: High quality RNA isolation from Aedes aegypti midguts using laser microdissection microscopy

   24 hour 48 hour
Gene Fixative 1 st 2 nd 1 st 2 nd
  Fresh 16.44 22.97 16.75 23.75
hsc70 Bouin's 19.50 32.45 22.49 27.44
  Carnoy's 16.98 16.34 16.59 17.20
  Fresh 27.32 34.87 26.22 34.94
ago1a & b Bouin's 30.50 37.77 33.61 37.91
  Carnoy's 26.81 27.26 25.43 28.63
  Fresh 23.17 27.57 22.34 27.79
ago2 Bouin's 24.77 30.35 27.04 28.36
  Carnoy's 22.84 23.90 23.11 23.58
  Fresh 24.78 28.07 24.93 28.62
hypo. protein Bouin's 25.66 33.36 31.70 29.27
  Carnoy's 24.43 24.94 26.00 27.03
  1. Each target mRNA was amplified using primers listed in Table 1 and total RNAs prepared from microdissected Aedes aegypti midgut tissues in two independent preparations (1st and 2nd).