Specificity analysis of three MLPA probes on agarose electrophoresis. All three probe pairs (CsPL for C.sinensis, OfPL for O.felineus, OvPL for O.viverrini) were added, different combined artificial DNA (Cs-T for C.sinensis, Ov-T for O.viverrini, Of-T for O.felineus) used as templates. The MLPA amplicons were separated in 5% agarose gel by electrophoresis and the image was taken under UV light. Fragment sizes: C.sinensis(198bp), O.felineus (170bp), O.viverrini(152bp). Lane M, 20 bp DNA ladder; Lane1, Cs-T+Ov-T+Of-T; Lane 2, Cs-T+Ov-T; lane 3, Cs-T+ Of-T; lane 4, Cs-T.