Molecular identification of Clonorchis sinensis and discrimination with other opisthorchid liver fluke species using multiple Ligation-depended Probe Amplification (MLPA)

Sun, Jiufeng; Xu, Jin; Liang, Pei; Mao, Qiang; Huang, Yan; Lv, Xiaoli; Deng, Chuanhuan; Liang, Chi; de Hoog, G S; Yu, Xinbing

doi:10.1186/1756-3305-4-98

Table 2 Evaluation of the three probe pairs in 66 different strains of C.sinensis isolates

From: Molecular identification of Clonorchis sinensis and discrimination with other opisthorchid liver fluke species using multiple Ligation-depended Probe Amplification (MLPA)

No.	GenBank	Source	CsPL+OfPL+OvPL+Ov-T+Of-T			CsPL+OfPL+OvPL+Of-T			CsPL+OfPL+OvPL
			198bp	170bp	152bp	198bp	170bp	152bp	198bp	170bp	152bp
1	HQ874538	Cat, Anhui, China	+	+	+	+	+	-	+	-	-
2	HQ874523	Cat, Anhui, China	+	+	+	+	+	-	+	-	-
3	HQ874584	Cat, Anhui, China	+	+	+	+	+	-	+	-	-
4	HQ874537	Cat, Anhui, China	+	+	+	+	+	-	+	-	-
5	HQ874599	Cat, Anhui, China	+	+	+	+	+	-	+	-	-
6	HQ874585	Cat, Anhui, China	+	+	+	+	+	-	+	-	-
7	HQ874586	Cat, Anhui, China	+	+	+	+	+	-	+	-	-
8	HQ874588	Cat, Anhui, China	+	+	+	+	+	-	+	-	-
9	HQ874540	Cat, Guangdong, China	+	+	+	+	+	-	+	-	-
10	HQ874535	Cat, Guangdong, China	+	+	+	+	+	-	+	-	-
11	HQ874541	Cat, Guangdong, China	+	+	+	+	+	-	+	-	-
12	HQ874602	Cat, Guangdong, China	+	+	+	+	+	-	+	-	-
13	HQ874587	Cat, Guangdong, China	+	+	+	+	+	-	+	-	-
14	HQ874532	Cat, Guangdong, China	+	+	+	+	+	-	+	-	-
15	HQ874581	Cat, Guangdong, China	+	+	+	+	+	-	+	-	-
16	HQ874582	Cat, Guangdong, China	+	+	+	+	+	-	+	-	-
17	HQ874542	Cat, Guangxi, China	+	+	+	+	+	-	+	-	-
18	HQ874536	Cat, Guangxi, China	+	+	+	+	+	-	+	-	-
19	HQ874543	Cat, Guangxi, China	+	+	+	+	+	-	+	-	-
20	HQ874529	Cat, Guangxi, China	+	+	+	+	+	-	+	-	-
21	HQ874580	Cat, Guangxi, China	+	+	+	+	+	-	+	-	-
22	HQ874533	Cat, Guangxi, China	+	+	+	+	+	-	+	-	-
23	HQ874525	Cat, Guangxi, China	+	+	+	+	+	-	+	-	-
24	HQ874579	Cat, Guangxi, China	+	+	+	+	+	-	+	-	-
25	HQ874544	Cat, Hubei, China	+	+	+	+	+	-	+	-	-
26	HQ874545	Cat, Hubei, China	+	+	+	+	+	-	+	-	-
27	HQ874593	Cat, Hubei, China	+	+	+	+	+	-	+	-	-
28	HQ874578	Cat, Hubei, China	+	+	+	+	+	-	+	-	-
29	HQ874539	Cat, Hubei, China	+	+	+	+	+	-	+	-	-
30	HQ874592	Cat, Hubei, China	+	+	+	+	+	-	+	-	-
31	HQ874546	Cat, Hubei, China	+	+	+	+	+	-	+	-	-
32	HQ874547	Cat, Hubei, China	+	+	+	+	+	-	+	-	-
33	HQ874524	Cat, Hubei, China	+	+	+	+	+	-	+	-	-
34	HQ874601	Cat, Henan, China	+	+	+	+	+	-	+	-	-
35	HQ874550	Cat, Henan, China	+	+	+	+	+	-	+	-	-
36	HQ874597	Cat, Henan, China	+	+	+	+	+	-	+	-	-
37	HQ874595	Cat, Henan, China	+	+	+	+	+	-	+	-	-
38	HQ874573	Cat, Henan, China	+	+	+	+	+	-	+	-	-
39	HQ874572	Cat, Henan, China	+	+	+	+	+	-	+	-	-
40	HQ874571	Cat, Henan, China	+	+	+	+	+	-	+	-	-
41	HQ874589	Cat, Henan, China	+	+	+	+	+	-	+	-	-
42	HQ874598	Cat, Hunan, China	+	+	+	+	+	-	+	-	-
43	HQ874590	Cat, Hunan, China	+	+	+	+	+	-	+	-	-
44	HQ874591	Cat, Hunan, China	+	+	+	+	+	-	+	-	-
45	HQ874551	Cat, Hunan, China	+	+	+	+	+	-	+	-	-
46	HQ874534	Cat, Hunan, China	+	+	+	+	+	-	+	-	-
47	HQ874552	Cat, Hunan, China	+	+	+	+	+	-	+	-	-
48	HQ874553	Cat, Hunan, China	+	+	+	+	+	-	+	-	-
49	HQ874554	Cat, Hunan, China	+	+	+	+	+	-	+	-	-
50	HQ874555	Dog, Jilin, China	+	+	+	+	+	-	+	-	-
51	HQ874556	Dog, Jilin, China	+	+	+	+	+	-	+	-	-
52	HQ874557	Dog, Jilin, China	+	+	+	+	+	-	+	-	-
53	HQ874570	Dog, Jilin, China	+	+	+	+	+	-	+	-	-
54	HQ874528	Dog, Jilin, China	+	+	+	+	+	-	+	-	-
55	HQ874527	Dog, Jilin, China	+	+	+	+	+	-	+	-	-
56	HQ874558	Cat, Jiangsu, China	+	+	+	+	+	-	+	-	-
57	HQ874566	Cat, Jiangsu, China	+	+	+	+	+	-	+	-	-
58	HQ874559	Cat, Jiangsu, China	+	+	+	+	+	-	+	-	-
59	HQ874530	Cat, Jiangsu, China	+	+	+	+	+	-	+	-	-
60	HQ874583	Cat, Jiangsu, China	+	+	+	+	+	-	+	-	-
61	HQ874569	Cat, Jiangsu, China	+	+	+	+	+	-	+	-	-
62	HQ874604	Cat, Jiangsu, China	+	+	+	+	+	-	+	-	-
63	HQ874565	Cat, Jiangxi, China	+	+	+	+	+	-	+	-	-
64	HQ874560	Cat, Jiangxi, China	+	+	+	+	+	-	+	-	-
65	HQ874561	Cat, Jiangxi, China	+	+	+	+	+	-	+	-	-
66	HQ874564	Cat, Jiangxi, China	+	+	+	+	+	-	+	-	-
67		Negative control	-	-	-	-	-	-	-	-	-

CsPL: Clonorchis sinensis Padlock probe pairs
OfPL: Opisthorchis felineus Padlock probe pairs
OvPL: Opisthorchis viverrini Padlock probe pairs
Cs-T: Artificial template of Clonorchis sinensis Padlock probe pairs
Ov-T: Artificial template of Opisthorchis viverrini Padlock probe pairs
Of-T: Artificial template of Opisthorchis felineus Padlock probe pairs

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ISSN: 1756-3305

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