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Table 2 Evaluation of the three probe pairs in 66 different strains of C.sinensis isolates

From: Molecular identification of Clonorchis sinensis and discrimination with other opisthorchid liver fluke species using multiple Ligation-depended Probe Amplification (MLPA)

No. GenBank Source CsPL+OfPL+OvPL+Ov-T+Of-T CsPL+OfPL+OvPL+Of-T CsPL+OfPL+OvPL
    198bp 170bp 152bp 198bp 170bp 152bp 198bp 170bp 152bp
1 HQ874538 Cat, Anhui, China + + + + + - + - -
2 HQ874523 Cat, Anhui, China + + + + + - + - -
3 HQ874584 Cat, Anhui, China + + + + + - + - -
4 HQ874537 Cat, Anhui, China + + + + + - + - -
5 HQ874599 Cat, Anhui, China + + + + + - + - -
6 HQ874585 Cat, Anhui, China + + + + + - + - -
7 HQ874586 Cat, Anhui, China + + + + + - + - -
8 HQ874588 Cat, Anhui, China + + + + + - + - -
9 HQ874540 Cat, Guangdong, China + + + + + - + - -
10 HQ874535 Cat, Guangdong, China + + + + + - + - -
11 HQ874541 Cat, Guangdong, China + + + + + - + - -
12 HQ874602 Cat, Guangdong, China + + + + + - + - -
13 HQ874587 Cat, Guangdong, China + + + + + - + - -
14 HQ874532 Cat, Guangdong, China + + + + + - + - -
15 HQ874581 Cat, Guangdong, China + + + + + - + - -
16 HQ874582 Cat, Guangdong, China + + + + + - + - -
17 HQ874542 Cat, Guangxi, China + + + + + - + - -
18 HQ874536 Cat, Guangxi, China + + + + + - + - -
19 HQ874543 Cat, Guangxi, China + + + + + - + - -
20 HQ874529 Cat, Guangxi, China + + + + + - + - -
21 HQ874580 Cat, Guangxi, China + + + + + - + - -
22 HQ874533 Cat, Guangxi, China + + + + + - + - -
23 HQ874525 Cat, Guangxi, China + + + + + - + - -
24 HQ874579 Cat, Guangxi, China + + + + + - + - -
25 HQ874544 Cat, Hubei, China + + + + + - + - -
26 HQ874545 Cat, Hubei, China + + + + + - + - -
27 HQ874593 Cat, Hubei, China + + + + + - + - -
28 HQ874578 Cat, Hubei, China + + + + + - + - -
29 HQ874539 Cat, Hubei, China + + + + + - + - -
30 HQ874592 Cat, Hubei, China + + + + + - + - -
31 HQ874546 Cat, Hubei, China + + + + + - + - -
32 HQ874547 Cat, Hubei, China + + + + + - + - -
33 HQ874524 Cat, Hubei, China + + + + + - + - -
34 HQ874601 Cat, Henan, China + + + + + - + - -
35 HQ874550 Cat, Henan, China + + + + + - + - -
36 HQ874597 Cat, Henan, China + + + + + - + - -
37 HQ874595 Cat, Henan, China + + + + + - + - -
38 HQ874573 Cat, Henan, China + + + + + - + - -
39 HQ874572 Cat, Henan, China + + + + + - + - -
40 HQ874571 Cat, Henan, China + + + + + - + - -
41 HQ874589 Cat, Henan, China + + + + + - + - -
42 HQ874598 Cat, Hunan, China + + + + + - + - -
43 HQ874590 Cat, Hunan, China + + + + + - + - -
44 HQ874591 Cat, Hunan, China + + + + + - + - -
45 HQ874551 Cat, Hunan, China + + + + + - + - -
46 HQ874534 Cat, Hunan, China + + + + + - + - -
47 HQ874552 Cat, Hunan, China + + + + + - + - -
48 HQ874553 Cat, Hunan, China + + + + + - + - -
49 HQ874554 Cat, Hunan, China + + + + + - + - -
50 HQ874555 Dog, Jilin, China + + + + + - + - -
51 HQ874556 Dog, Jilin, China + + + + + - + - -
52 HQ874557 Dog, Jilin, China + + + + + - + - -
53 HQ874570 Dog, Jilin, China + + + + + - + - -
54 HQ874528 Dog, Jilin, China + + + + + - + - -
55 HQ874527 Dog, Jilin, China + + + + + - + - -
56 HQ874558 Cat, Jiangsu, China + + + + + - + - -
57 HQ874566 Cat, Jiangsu, China + + + + + - + - -
58 HQ874559 Cat, Jiangsu, China + + + + + - + - -
59 HQ874530 Cat, Jiangsu, China + + + + + - + - -
60 HQ874583 Cat, Jiangsu, China + + + + + - + - -
61 HQ874569 Cat, Jiangsu, China + + + + + - + - -
62 HQ874604 Cat, Jiangsu, China + + + + + - + - -
63 HQ874565 Cat, Jiangxi, China + + + + + - + - -
64 HQ874560 Cat, Jiangxi, China + + + + + - + - -
65 HQ874561 Cat, Jiangxi, China + + + + + - + - -
66 HQ874564 Cat, Jiangxi, China + + + + + - + - -
67   Negative control - - - - - - - - -
  1. CsPL: Clonorchis sinensis Padlock probe pairs
  2. OfPL: Opisthorchis felineus Padlock probe pairs
  3. OvPL: Opisthorchis viverrini Padlock probe pairs
  4. Cs-T: Artificial template of Clonorchis sinensis Padlock probe pairs
  5. Ov-T: Artificial template of Opisthorchis viverrini Padlock probe pairs
  6. Of-T: Artificial template of Opisthorchis felineus Padlock probe pairs