Figure 5
From: The role of Src kinase in the biology and pathogenesis of Acanthamoeba castellanii
Inhibition of Src kinase had no effect on A. castellanii adhesion to and cytotoxicity of HBMEC. (A) Various concentrations of PP2 or PP3 were added to A. castellanii (4 x 105 amoebae/well) for 30 min. After this incubation, A. castellanii were washed with PBS, and added to HBMEC monolayers for 1 h. The unbound amoebae were counted using a haemocytometer and the numbers of bound amoebae were deduced as follows: No. of unbound amoebae/Total number of amoebae x 100 = % unbound amoebae. The numbers of bound amoebae were deduced as follows: % unbound amoebae – 100 = % bound amoebae. (B) As for adhesion assays, amoebae were incubated with HBMEC for up to 24 h and the percent HBMEC cytotoxicity was determined by measuring LDH release. Results represent the mean of three independent experiments performed in duplicate. Bars represent standard error.