Table 2 MBN-base/MBN-DDT primer information for qPCR (F = forward, R = reverse)
Gene | Primer sequence | Annealing temperature | Amplicon length |
|---|---|---|---|
CYP6M2 | F 5’- CAT GAC ACA AAC CGA CAA GG -3’ | 60.0°C | 235 |
|  | R 5’- GGT GAG GAG AGT CGA CGA AG -3’ |  |  |
CYP6AK1 | F 5’- TCA TCG AGC GAC AGT GTA CC -3’ | 58.3°C | 251 |
|  | R 5’- AAA GTG TGA CCC CAG ACA GG -3’ |  |  |
CYP6P3 | F 5’- CGA TTC TTC CTG GAC ATC GT -3’ | 58.3°C | 141 |
|  | R 5’- CTT GCC CAA ACT ACC GTC AT -3’ |  |  |
TPX4 | F 5’- CAG CTG ACA GAC CGA TTA AG -3’ | 58.3°C | 116 |
|  | R 5’- CCG TTC GGG AAC AGT TTG TCT -3’ |  |  |
β-actin | F 5’- ACC AAG AGC CTG AAG CAC -3’ | * | 123 |
|  | R 5’- CGA GCA CGA CAC ACT ATA TAC -3’ |  |  |