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Table 1 Primers, sequences, and sizes of PCR products used in the molecular identification of Anopheles minimus complex species present in Thailand

From: Host feeding patterns and preference of Anopheles minimus (Diptera: Culicidae) in a malaria endemic area of western Thailand: baseline site description

Species Primer name Sequence (5’ to 3’) Size of the product (bp) Tm (oC)
Universal forward primer ITS2 TGT GAA CTG CAG GAC ACA T   54.5
Anopheles pampanai PAM TGT ACA TCG GCC GGG GTA 90 56.0
Anopheles aconitus ACO ACA GCG TGT ACG TCC AGT 200 58.2
Anopheles harrisoni MIC GTT CAT TCA GCA ACA TCA GT 180 53.2
Anopheles varuna VAR TTG ACC ACT TTC GAC GCA 260 53.7
Anopheles minimus MIA CCC GTG CGA CTT GAC GA 310 57.6
  1. *The internal transcribed spacer 2 (ITS2A) is the universal primer that binds to the same position on the ITS2 DNA for all 5 species, while the specific primers (PAM to MIA) bind at different places on the ITS2 DNA of the corresponding species. bp = number base pairs; Tm = melting temperature.