Species
|
Primer name
|
Sequence (5’ to 3’)
|
Size of the product (bp)
|
Tm (oC)
|
---|
Universal forward primer
|
ITS2
|
TGT GAA CTG CAG GAC ACA T
| |
54.5
|
Anopheles pampanai
|
PAM
|
TGT ACA TCG GCC GGG GTA
|
90
|
56.0
|
Anopheles aconitus
|
ACO
|
ACA GCG TGT ACG TCC AGT
|
200
|
58.2
|
Anopheles harrisoni
|
MIC
|
GTT CAT TCA GCA ACA TCA GT
|
180
|
53.2
|
Anopheles varuna
|
VAR
|
TTG ACC ACT TTC GAC GCA
|
260
|
53.7
|
Anopheles minimus
|
MIA
|
CCC GTG CGA CTT GAC GA
|
310
|
57.6
|
- *The internal transcribed spacer 2 (ITS2A) is the universal primer that binds to the same position on the ITS2 DNA for all 5 species, while the specific primers (PAM to MIA) bind at different places on the ITS2 DNA of the corresponding species. bp = number base pairs; Tm = melting temperature.