Host feeding patterns and preference of Anopheles minimus (Diptera: Culicidae) in a malaria endemic area of western Thailand: baseline site description

Table 1 Primers, sequences, and sizes of PCR products used in the molecular identification of Anopheles minimus complex species present in Thailand

Species	Primer name	Sequence (5’ to 3’)	Size of the product (bp)	Tm (^oC)
Universal forward primer	ITS2	TGT GAA CTG CAG GAC ACA T		54.5
Anopheles pampanai	PAM	TGT ACA TCG GCC GGG GTA	90	56.0
Anopheles aconitus	ACO	ACA GCG TGT ACG TCC AGT	200	58.2
Anopheles harrisoni	MIC	GTT CAT TCA GCA ACA TCA GT	180	53.2
Anopheles varuna	VAR	TTG ACC ACT TTC GAC GCA	260	53.7
Anopheles minimus	MIA	CCC GTG CGA CTT GAC GA	310	57.6

*The internal transcribed spacer 2 (ITS2A) is the universal primer that binds to the same position on the ITS2 DNA for all 5 species, while the specific primers (PAM to MIA) bind at different places on the ITS2 DNA of the corresponding species. bp = number base pairs; Tm = melting temperature.

ISSN: 1756-3305