Figure 3

The kDNA PCR and RFLP products of the 12 Palestinian L. tropica strains. a, The kDNA PCR products of three of the 12 Palestinian strains: LRC-L890, -L891, -L887, and reference strains of L. tropica, -L758, and L. major, -L137; MW: molecular weight marker ΦX174 DNA/Hinf; b, The RFLP patterns of the six different genotypes resulting from digestion of kDNA PCR products with Rsa I. MW: molecular weight marker ΦX174 DNA/HaeIII Markers; c, Dendrogram based on the kDNA restriction fragment length polymorphism (RFLP) of the strains of L. tropica after digestion of their PCR products, separately, with the endonucleases Rsa I and Mbo I, which yielded ten sub-genotypes that segregated into the Clade A, with kDNA sub-type Ltro-kD1 and the Clade B, with kDNA sub-type Ltro-kD2. It was constructed using RAPDistance Package (version 1.04) and was based on the presence or absence of the bands in each sample. The numbers on the branches represent the % of difference. The lengths of the branches indicate the degree of similarity in fingerprints among the strains of L. tropica relative to the strain of L. major, MHOM/IL/67/JerichoII (=LRC-L137), which served as an out-group in this analysis: Ts = Tubas; Ma = Meselya; Yn = El-Yamoon; Jn = Jenin; S-h = Silat El hartheyia; Tb = Tiberias; KA = Kfar Adumim. The zymodemal designations and EF serotypes are given for complete comparison.