Genetic, serological and biochemical characterization of Leishmania tropica from foci in northern Palestine and discovery of zymodeme MON-307

Table 1 The strains of Leishmania used in this study

WHO code	LRC#	Geographic origin	Microsatellite		Clade kDNA^b	EFserotype^c	Zymodeme^d
			Cluster^a	Subgp
MHOM/PS/2002/18JnF4*	L891	Meselya	II	B1a	A Ltro-kD1/3	A2	MON-307
MHOM/PS/2002/20JnM3*	L884	El-Yamoon	II	B1b	A Ltro-kD1/2	A2	MON-307
MHOM/PS/2002/52JnM18*	L890	El-Yamoon	II	B1b	A Ltro-kD1/1	A2	MON-307
MHOM/PS/2002/5JnF5*	L893	El-Yamoon	I	A1b	B Ltro-kD2/2	A9	MON-137
MHOM/PS/2002/87JnM*	L887	El-Yamoon	I	A1b	B Ltro-kD2/5	A9B4	MON-137
MHOM/IL/1997/P963*	L725	Tiberias, Israel	I	A1a	B Ltro-kD2/1	A9B4	MON-137
MHOM/PS/2002/79JnF20*	L885	Silat El hartheyia	I	A1a	B Ltro-kD2/6	A9B4	MON-137
MHOM/PS/2002/89JnF*	L886	Silat El hartheyia	I	A1a	B Ltro-kD2/6	A9B4	MON-137
MHOM/PS/2002/64JnF4*	L889	Silat El hartheyia	I	A1b	B Ltro-kD2/6	A9B4	MON-137
MHOM/PS/2002/34JnF4*	L882	Silat El hartheyia	I	A1b	B Ltro-kD2/3	A9B4	MON-137
ISER/IL/1998/LRC-L758*	L758	Kfar Adumim, Israel	I	A1b	B Ltro-kD2/1	A9B4	MON-137
MHOM/PS/2002/31JnM17	L883	Tubas	nd		B Ltro-kD2/6	A9B4	MON-137
MHOM/PS/2008/336JnM71	L1324	Silat El hartheyia	nd		B Ltro-kD2/4	A9B4	nd
MHOM/PS/2002/35JnF45	L888	Jenin City	nd		B Ltro-kD2/6	A9	nd
MHOM/IL/1967/JerichoII	L137	Near Jericho	Lmj 39		Lmaj-kD1	A1	MON-26

* Strains previously analysed by multilocus microsatellite typing (MLMT) using 21 microsatellite markers from Schwenkenbecher [3]^a, their microsatellite cluster (I and II) , Subgroup (Subgp) as delineated here (Figure 2); ^b, kDNA PCR-RFLP; ^c, excreted factor (EF) serotype done according to Schnur and Zuckerman [15] ; ^d, MLEE done according to Rioux [18] and Pratlong [19] ), and of L. major MOHM/IL/1967/JerichoII (=LRC-L137) using 10 microsatellite markers and taken from Al Jawabreh [39]. The Israeli strains of L. tropica LRC-L725 and LRC-L758 were used as reference strains of L. tropica; nd, not done.

ISSN: 1756-3305