Figure 2

Genetic distance tree of ace-1 sequences from C. pipiens carrying a duplicated haplotype. The similarity of the various duplicated ace-1 alleles was computed using the Neighbor-Joining method and the genetic distance tree is shown. Sequences of intron 2 and exon 3 were used and positions containing gaps and missing data were eliminated. There were a total of 502 positions in the final dataset. These analyses were conducted in MEGA5 [26]. D₁₃(S) and D₁₃(R) copies of the Lebanon duplicated allele are in bold characters. Geographical origins of each duplicated allele are indicated (Fr: France; Alg: Algeria; Phil: Philippines, Mart: Martinique).