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Figure 1 | Parasites & Vectors

Figure 1

From: Participation of heparin binding proteins from the surface of Leishmania (Viannia) braziliensis promastigotes in the adhesion of parasites to Lutzomyia longipalpis cells (Lulo) in vitro

Figure 1

Denaturant electrophoresis assays with Leishmania (V.) braziliensis proteins. Protein samples (10 μg) of flagella (Ff) and membrane (Mf) fractions collected prior to heparin-sepharose column fractionation (1 and 3, respectively) or afterwards (HBP F f - 2 and HBP M f - 4) were applied into each slot, submitted to SDS-PAGE and revealed by silver staining (A). In parallel, protein samples (10 μg) from parasite cell lysates (5 and 6), F f (7) and M f (8) were separated by SDS-PAGE, transferred to a nitrocellulose membrane and revealed by Western blotting assays (B). In this last case, subcellular markers as Na+/K + ATPase (80 kDa) and GAPDH (36 kDa) proteins (arrows) were detected using specific antibodies and an HRP-conjugated secondary antibody. Bands were visualized by chemiluminescence. These results are representative of four independent experiments.

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