Figure 1From: Participation of heparin binding proteins from the surface of Leishmania (Viannia) braziliensis promastigotes in the adhesion of parasites to Lutzomyia longipalpis cells (Lulo) in vitroDenaturant electrophoresis assays with Leishmania (V.) braziliensis proteins. Protein samples (10 μg) of flagella (Ff) and membrane (Mf) fractions collected prior to heparin-sepharose column fractionation (1 and 3, respectively) or afterwards (HBP F f - 2 and HBP M f - 4) were applied into each slot, submitted to SDS-PAGE and revealed by silver staining (A). In parallel, protein samples (10 μg) from parasite cell lysates (5 and 6), F f (7) and M f (8) were separated by SDS-PAGE, transferred to a nitrocellulose membrane and revealed by Western blotting assays (B). In this last case, subcellular markers as Na+/K + ATPase (80 kDa) and GAPDH (36 kDa) proteins (arrows) were detected using specific antibodies and an HRP-conjugated secondary antibody. Bands were visualized by chemiluminescence. These results are representative of four independent experiments.Back to article page