Figure 2

Quantification and identification of sulfated glycosaminoglycans in Lulo cells. After metabolic labeling of Lulo cells with ³⁵ S-Na₂SO₄, cell extract, cell surface and cell culture medium were subjected to enzymatic degradation and afterwards to gel electrophoresis in 0.6% agarose. The bands were identified using an image analysis system, the Cyclone® Storage Phosphor System-Packard Instrument (A). Quantification was performed by densitometry with Opti-Quanti Software and expressed as cpm/mg protein (B). Defined quantities of non-labeled GAGs (chondroitin sulfate - CS; heparan sulfate - HS) were used as standard control; CTRL – incubation control with buffer; AC – chondroitinase AC; ABC – chondroitinase ABC; HEP II – heparinase II.