Figure 3From: Participation of heparin binding proteins from the surface of Leishmania (Viannia) braziliensis promastigotes in the adhesion of parasites to Lutzomyia longipalpis cells (Lulo) in vitroQuantification of promastigote adhesion to Lulo cells. Adhesion inhibition assays were performed using promastigotes without or with pre-incubation with increasing concentrations of heparan sulfate (A) were co-incubated (28°C) with Lulo cells monolayers. Likewise, Lulo cells monolayers without or with pre-incubation with different concentrations (10 μg and 20 μg) of HBP F f and HBP Mf, and 20 μg of BSA (control) were co-incubated with promastigotes (B). The coverslip containing cells and/or parasites samples were stained with Giemsa and the number of adhered promastigotes/100 cells was determined by light microscopy examining 300 cells per coverslip, in triplicate. Data are expressed as percentile values (%) and represent average and standard deviation of five independent experiments - (*), p < 0.05.Back to article page