Melting curve analyses of kDNA conserved regions amplicons. Representative SYBR Green dissociation curves of kDNA amplified products to determinate the melting temperature values [Tm]. Following amplification in the ABI Prism 7500 Sequence Detection System, the amplicons were submitted to a gradual temperature increase (0.1°C/s to 90°C). The Tm calculation of each amplicon was done directly by the software provided (indicated by the perpendicular line in the graphics). Each trace in the charts represents a single analyzed DNA sample. The graphics on the left represent typical kinetic dissociation profiles obtained for the Viannia subgenus: (A1) L. (V.) shawi and L. (V.) braziliensis promastigote reference strains; (B1) clinical samples from cutaneous disease patients; (C1) Lu. intermedia and Lu. migonei pools naturally infected by L. (V.) braziliensis. On the right are represented the melting profiles for the subgenus Leishmania: (A2) L. (L.) amazonensis and L. (L.) infantum promastigote reference strains; (B2) clinical specimens from visceral disease patients; (C2) Lu. cruzi and Lu. forattinii pools naturally infected by L. (L.) infantum.