Sequence alignment of amplified minicircles kDNA conserved region and phylogenetic analysis. A - Alignment of 12 generated sequences representing three clones for each analyzed Leishmania reference strain. Highlighted in gray: Exclusively L. (Viannia) subgenus conserved nucleotides. Most of the substitutions shown are possibly associated with the lower melting temperature (Tm) characteristic for this subgenus (indicated by asterisks). B - Neighbor-Joining tree based on kDNA amplicons sequence. The rectangles represent sequences from subgenus Viannia and circles indicate those from subgenus Leishmania. Bootstraps values are indicated.