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Figure 2 | Parasites & Vectors

Figure 2

From: SYBR Green-based Real-Time PCR targeting kinetoplast DNA can be used to discriminate between the main etiologic agents of Brazilian cutaneous and visceral leishmaniases

Figure 2

Sequence alignment of amplified minicircles kDNA conserved region and phylogenetic analysis. A - Alignment of 12 generated sequences representing three clones for each analyzed Leishmania reference strain. Highlighted in gray: Exclusively L. (Viannia) subgenus conserved nucleotides. Most of the substitutions shown are possibly associated with the lower melting temperature (Tm) characteristic for this subgenus (indicated by asterisks). B - Neighbor-Joining tree based on kDNA amplicons sequence. The rectangles represent sequences from subgenus Viannia and circles indicate those from subgenus Leishmania. Bootstraps values are indicated.

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Parasites & Vectors

ISSN: 1756-3305

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