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Figure 5 | Parasites & Vectors

Figure 5

From: Molecular characterization of thyroid hormone receptor beta from Schistosoma japonicum and assessment of its potential as a vaccine candidate antigen against schistosomiasis in BALB/c mice

Figure 5

Western blot. ( A) SDS–PAGE analysis of the rSjTHRβ-DBD protein. Lane1: Purified rSjTHRβ-DBD protein (about 22 kDa). Lane2: Soluble fractions of expressed product of pET28a (+)-SjTHRβ-DBD induced with IPTG of the E. coli extract. Lane3: Expressed product of pET28a (+)-SjTHRβ-DBD induced with IPTG. Lane4: Expressed product of pET28a (+)-THRβ-DBD without induction. ( B) SDS–PAGE analysis of the rSjTHRβ-LBD protein. Lane1: Expressed product of pET32a (+)-SjTHRβ-LBD without induction. Lane2: Expressed product of pET32a (+)-SjTHRβ-LBD induced with IPTG. Lane3: Soluble fractions of expressed product of pET32a (+)-SjTHRβ-LBD induced with IPTG of the E.coli extract. Lane4: Insoluble fractions of expressed product of pET32a (+)-SjTHRβ-LBD induced with IPTG of the E.coli extract. Lane5: Purified rSjTHRβ-LBD protein (about 45 kDa) ( C) The protein extract of 21d schistosomes (lane1) and E.coli BL21 (DE3) (lane2) were probed with mouse serum specific to rSjTHRβ-LBD. The blots are representative of three replicates. M: protein marker.

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Parasites & Vectors

ISSN: 1756-3305

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