Skip to main content
Figure 6 | Parasites & Vectors

Figure 6

From: Molecular characterization of thyroid hormone receptor beta from Schistosoma japonicum and assessment of its potential as a vaccine candidate antigen against schistosomiasis in BALB/c mice

Figure 6

rSjTHRβ-DBD protein binds to Half-site and DR0 in vitro . ( A) Binding of rSjTHRβ-DBD protein to DR0. Lane1: Reaction mixture without rSjTHRβ-DBD as negative control. Lane2: Contains rSjTHRβ-DBD. Lane3: Contains rSjTHRβ-DBD with 100 fold of Specific competitive probe (SCP). Lane4: Contains rSjTHRβ-DBD with 100 fold of Non specific competitive probe (Non-SCP) ( B) Binding of rSjTHRβ-DBD protein to Half-site (HS). Lane1: Contains rSjTHRβ-DBD with 100 fold of Non specific competitive probe (Non-SCP). Lane2: Contains rSjTHRβ-DBD with 100 fold of Specific competitive probe (SCP). Lane3: Contains rSjTHRβ-DBD. Lane4: Reaction mixture without rSjTHRβ-DBD as negative control. ( C) Super-shift experiment. Lane1: Probe DR0 without anti-rSjTHRβ-DBD Serum. Lane2: Probe DR0 with anti-rSjTHRβ-DBD Serum. Lane3: Probe HS with anti-rSjTHRβ-DBD Serum. Lane4: Probe HS without anti-rSjTHRβ-DBD Serum.

Back to article page

Parasites & Vectors

ISSN: 1756-3305

Contact us