Table 1 Primer sets and real-time PCR conditions used for analysis of differentially expressed genes
Gene description | Genbank accession number | Upstream/downstream primer sequences (5´-3´) | PCR conditionsa |
|---|---|---|---|
Interleukin 1 receptor accessory protein-like 1 (IL1RAPL1) | NG_008292 CN163387 | IL1-L: GTTGTCATTTCGCCAAACCT IL1-R: GCCTATGACCGATGGCTTTA | 58°C, 30 sec/72 °C, 30 sec |
T-cell receptor alpha chain (TCR-alpha) | AB087958.1 | TcellR-L: TTCTGACCCTGGGGACTATG TcellR-R: GAGAAGCCATGCTGTTGGT | 58°C, 30 sec/72°C, 30 sec |
Gap junction protein alpha 1 (GJA1) | BC105464.1 CK465005 | GAP-L: TGCAATGAAGCTGAACATGA GAP-R: TGGAATGCAAGAGAGGTTGA | 58°C, 30 sec/72°C, 30 sec |
Thrombospondin 4 (TSP-4) | XM_001926236 BM190304 | TROMB4-L: GGGCAAGGTTTTTGTTCTGA TROMB4-R: TCATAGGGGTCCAGCACTTC | 60°C, 30 sec/72°C, 30 sec |
Beta-actin | DQ845171 | SusBetActin-L: GACATCCGCAAGGACCTCTA SusBetActin-R: ACACGGAGTACTTGCGCTCT | 60°C, 30 sec/72°C, 30 sec |
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) | AF069649 | GADPHSus-L: CCAGAACATCATCCCTGCTT GADPHSus-R: GTCCTCAGTGTAGCCCAGGA | 60°C, 30 sec/72 °C, 30 sec |
Cyclophilin | AY008846 | SSCYCLOPHILIN-L: AGCACTGGGGAGAAAGGATT SSCYCLOPHILIN-R: CTTGGCAGTGCAAATGAAAA | 55 °C, 30 sec/72 °C, 30 sec |