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Figure 2 | Parasites & Vectors

Figure 2

From: Serological and molecular survey of Leishmania parasites in apparently healthy dogs in the West Bank, Palestine

Figure 2

Detection of Leishmania using agarose gel electrophoresis. ITS1 and CPB-PCR products generated from amplification of DNA in buffy coat of dogs from the West Bank, Palestine. (a) ITS1-PCR produced a single band (310 bp) diagnostic for Leishmania DNA. 100 bp; DNA size marker, lanes from 115–133 represent dog samples, numbers with (+) represent inhibition control ( L. turanica DNA added to sample). Lane 124 represent a positive sample, (−) represent negative control, and (+) represent positive control. (b) Identification of Leishmania isolated from a dog with CVL by CPB- PCR. 100 bp DNA size marker, 187; sample MCAN/PS/2010/LRUJ-187 isolated from asymptomatic dog. Inf; L. infantum (positive control), don; L. donovani (positive control) and (−) negative control.

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Parasites & Vectors

ISSN: 1756-3305

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