Work flow for profiling of small RNAs after high-through-put sequencing. Approaches and numbers of sequence tags obtained after each step were shown for both strains. The simple pie chart shows the number of strain-specific and common miRNAs identified in the two libraries. More miRNAs (155) were identified in RH strain than that in ME49. The number of miRNAs that commonly expressed in the two strains is 181.