Characterization of four miRNAs by Northern-blot. Lanes from left to right are RNAs from ME49 strain (ME49), RH strain (RH) and a mouse. Total RNA isolation from ME49, RH strain and a mouse were visualized by ethidium bromide staining and served as loading controls at the top panel. LNA probes corresponding to tgo-Novel-1-1-3p, tgo-Novel-12-1, tgo-Novel-40, tgo-Novel-41, tgo-Novel-14-2 were used. Probe tgo-Novel-1-1-3p and tgo-Novel-40 only hybridized to the RNAs of RH strain and ME49, respectively. While probes of tgo-Novel-12-1, tgo-Novel-41 and tgo-Novel-14-2 hybridized to RNA of both strains. More hybridization was seen with tgo-Novel-41 probe to ME49. More hybridization was seen with pre-miRNAs except with the probe of tgo-Novel-41. No hybridization was detected with mouse RNA with any probe. Hybridization to mature miRNAs was marked with asterisks.