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Table 2 Previously identified infection rates with Anaplasma phagocytophilum in questing Ixodes ricinus in Germany

From: Babesia spp. and Anaplasma phagocytophilum in questing ticks, ticks parasitizing rodents and the parasitized rodents – Analyzing the host-pathogen-vector interface in a metropolitan area

Year of tick collection

Location in Germany

No. of ticks

Average prevalence in%

Method

Target gene

Reference

2003

North

127b

3.9

Reverse Line Blot

16S rRNA

[82]

2005

North

1,646c

3.2

Real-time PCR

Msp2 a

[85]

2003

Central

305c

2.3

Conventional PCR

16S rRNA

[86]

2006-2007

Central

1,000c

5.4

Conventional PCR

16S rRNA

[73]

2007

Central

196c

0

Conventional PCR

16S rRNA

[79]

1999

South

492c

1.6

Conventional PCR

16S rRNA

[87]

1999-2001

South

5,424

1.9

Nested PCR

16S rRNA

[78]

2002

South

1,963

2.8

Nested PCR

16S rRNA

[88]

2003-2004

South

625

4.5

Real-time PCR

Msp2 a

[84]

2006

South

2,862c

2.9

Real-time PCR

Msp2 a

[29]

2009-2010

South

5,569c

9.0

Real-time PCR

Msp2 a

[53]

1998 -2010

Total

20,209

4.3

   
  1. a multicopy target.
  2. b only nymphs.
  3. c study incl. nymphs.