Analysis results of 9 blood samples from T. gondii infected mice. (A) 529 bp- nested PCR; (B) 529 bp- LAMP. I, II, III represent three groups of BALB/c mice (n = 3 mice/group) that were infected by intraperitoneal injection with 20 virulent tachyzoites of T. gondii RH strain. Lane M represents 2,000 bp DNA ladder; lane Nc represents negative control; lane Ic represents internal control; lane Pc represents positive control; lanes 1-9 represent sample ID. Blood samples from mice were collected at 1, 3, and 5 days post injection.