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Figure 2 | Parasites & Vectors

Figure 2

From: PCR identification of culicoid biting midges (Diptera, Ceratopogonidae) of the Obsoletus complex including putative vectors of bluetongue and Schmallenberg viruses

Figure 2

Validation of species-specific PCR primers. Lanes 1, 19: 100 bp DNA ladder, lane 2: negative control (no template), lanes 3, 7, 11, 15: C. obsoletus, lanes 4, 8, 12, 16: C. scoticus, lanes 5, 9, 13, 17: C. chiopterus, lanes 6, 10, 14, 18: C. dewulfi. The specific primers used were obs-COI-fwd (lanes 2-6), sco-COI-fwd (lanes 7-10), chio-COI-fwd (lanes 11-14), and dew-COI-fwd (lanes 15-18) – all in combination with the universal reverse primer PanCuli-COX1-727R.

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Parasites & Vectors

ISSN: 1756-3305

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