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Table 3 Suggested species-specific primers

From: PCR identification of culicoid biting midges (Diptera, Ceratopogonidae) of the Obsoletus complex including putative vectors of bluetongue and Schmallenberg viruses

Species

Primer code

Primer sequence (5’-3’)

Product length (bp)

C. dewulfi

dew-COI-fwd

CGCCCGACATAGCATTCCCT

468

C. obsoletus

obs-COI-fwd

CAGGAGCTTCTGTAGATTTGGCT

318

C. scoticus

sco-COI-fwd

CCACAATTATTAATATGCGATCTACC

237

C. chiopterus

chio-COI-fwd

CCTTTATTTGTTTGGTCTGTTCTTC

190

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Parasites & Vectors

ISSN: 1756-3305

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