Figure 4

Bioluminescent imaging of Rhodnius prolixus infected with Dm28c-luc . On the seventh day after feeding, all insects were evaluated by bioluminescent emission in vivo, after D-luciferin injection into the hemocoel, and divided in four groups based on the radiance measured, that was predominant in the posterior region of the insect (A). In contrast, the ex vivo bioluminescent imaging from the distensible crop of the dissected digestive tract, displayed intense bioluminescence in the crop, corroborated by parasite quantification in a Neubauer chamber, as displayed in the enlarged images (B).