Phylogenetic tree of the positive sequences based on 243 nucleotides of partial NS5 gene. The tree was displayed by using the program Mega 5.05, Neighbor-Joining method and distance-p model with 1000 bootstrap replicates. The consensus flavivirus sequence obtained in the current study (AEFV Italy 2008) is in bold in the tree. The GenBank accession numbers for the AEFV sequences obtained in this work are JX860634, JX860635, JX860636 and JX860637. AEFV: Aedes flavivirus, KRV: Kamiti River virus, CXFV: Culex flavivirus, CFA: Cell fusing agent and TABV: Tamana bat virus as outgroup.