Figure 3

Thioredoxin silencing in E. histolytica reduce the Erythrocytes and E. coli phagocytosis. Entamoeba histolytica trophozoites were preincubated in the presence or absence of 50, 100 or 250 μg of PATMK and thioredoxin siRNA during 15 h. a) representative RT-PCR bands of PAMTK and thioredoxin amplified products of silencing and non-silencing trophozoites. b) Densitometric analysis RT-PCR products normalized to PATMK/Actin or Thioredoxin/Actin density of non-silenced trophozoites. c) Erythrophagocytosis of siRNA PATMK and thioredoxin silenced trophozoites. d) GFP-labeled E. coli phagocytosis of siRNA PATMK or thioredoxin silenced trophozoites (MIF: Median Intensity Fluorescence). Results were analyzed using Repeated-measures one-way ANOVA and Bonferroni test. Significance was set at P < 0.05.