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Table 3 MALDI-TOF MS based species identification of 100 randomly selected Culicoides specimens from the 12 trapping sites 1

From: Spatio-temporal occurrence of Culicoides biting midges in the climatic regions of Switzerland, along with large scale species identification by MALDI-TOF mass spectrometry

Site Culicoides species (no.) {no. confirmed by PCR}3[%4]
(altitude2) Morphological group Unknown Genetic identification4 No identification5
  Obsoletus Pulicaris OtherCulicoides
Novazzano C. obsoletus (84) {5} C. grisescens II (1) C. circumscriptus (4) {1} 0 0
(270) C. scoticus (5) C. pulicaris (1) C. pallidicornis (4)   
   C. punctatus (1)    
  [91.4] [1.9] [6.7]   
Dittingen C. obsoletus (3) C. lupicaris (2) 0 0 0
(360) C. scoticus (95) {6}     
  [99.9] [0.1] [0]   
Commugny C. obsoletus (91) {6} 0 0 C. kibunensis (2) 3
(420) C. scoticus (4)     
  [98.4] [0] [1.6]   
Granges C. obsoletus (88) {6} C. grisescens II (1) 0 0 0
(500) C. scoticus (9) C. pulicaris (2)    
  [100] [0] [0]   
Wädenswil C. obsoletus (91) {5} C. lupicaris (5) {1} C. pallidicornis (1) 0 0
(620) C. scoticus (2) C. pulicaris (1)    
  [95] [4.2] [0.8]   
Mühlethurnen C. obsoletus(74) {4} C. lupicaris (1) 0 C. segnis (1) 3
(670) C. scoticus (19) {1}     
  C. dewulfi (2) {1}     
  [99.0] [1.0] [0]   
Bennau C. obsoletus (74) {4} C. grisescens II (1) {1} C. pallidicornis (1) 0 4
(810) C. scoticus (11) {1} C. lupicaris (9)    
  [92.1] [5.6] [2.2]   
Château d’Oex C. obsoletus (91) {6} C. lupicaris (1) 0 C. newsteadi (1) 0
(940) C. scoticus (3) C. pulicaris (3)    
   C. punctatus (1)    
  [90.6] [4.7] [4.7]   
Chaumont C. obsoletus (12) {2} C. lupicaris (1) 0 0 3
(1,110) C. scoticus (75) {3} C. pulicaris (1)    
  C. chiopterus (6) C. punctatus (1)    
  C. dewulfi (1) {1}     
  [94.2] [1.9] [3.9]   
Davos C. obsoletus (5) C. grisescens I (4) {1} C. pallidicornis (1) 0 0
(1,560) C. scoticus (2) C. grisescens II (87) {5}    
   C. pulicaris (1)    
  [4.2] [95.0] [0.8]   
Sils C. obsoletus (4) C. deltus (6) {1} 0 C. fascipennis (2) 0
(1,800) C. scoticus (3) C. grisescens I (5)   C. reconditus (1)  
   C. grisescens II (75) {5}    
   C. pulicaris (4)    
  [4.5] [82.9] [12.6]   
Juf   C. grisescens I (51) {4} 0 C. fascipennis (3) 0
(2,130)   C. grisescens II (40) {2}   C. reconditus (2)  
   C. pulicaris (2)   C. segnis-like (2)6  
  [1.5] [94] [4.5]   
  1. 1 Specimens randomly selected from a single catch from the high abundance season (August/September 2010), stored in 70% ethanol for more than 1 year and analysed using a database containing validated biomarker mass sets of 15 Culicoides species[27].
  2. 2 Meters above sea level.
  3. 3 PCR/sequencing[22] with 6 randomly chosen (http://www.random.org) specimens/site. Species identification by comparison with GenBank entries (>99% identity).
  4. 4 Percentage of Culicoides morphologically classified as belonging to Obsoletus group, Pulicaris group or other Culicoides of the catch from which the specimens were selected for the MALDI-TOF MS analyses.
  5. 4 Identified by PCR/sequencing[22].
  6. 5 No species identification achievable due to poor quality mass spectra.
  7. 6 Best match: 96% identity (403/422) with GenBank entry HQ824509 (Ccxx segnis from Switzerland).