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Table 3 MALDI-TOF MS based species identification of 100 randomly selected Culicoides specimens from the 12 trapping sites 1

From: Spatio-temporal occurrence of Culicoides biting midges in the climatic regions of Switzerland, along with large scale species identification by MALDI-TOF mass spectrometry

Site

Culicoides species (no.) {no. confirmed by PCR}3[%4]

(altitude2)

Morphological group

Unknown Genetic identification4

No identification5

 

Obsoletus

Pulicaris

OtherCulicoides

Novazzano

C. obsoletus (84) {5}

C. grisescens II (1)

C. circumscriptus (4) {1}

0

0

(270)

C. scoticus (5)

C. pulicaris (1)

C. pallidicornis (4)

  
  

C. punctatus (1)

   
 

[91.4]

[1.9]

[6.7]

  

Dittingen

C. obsoletus (3)

C. lupicaris (2)

0

0

0

(360)

C. scoticus (95) {6}

    
 

[99.9]

[0.1]

[0]

  

Commugny

C. obsoletus (91) {6}

0

0

C. kibunensis (2)

3

(420)

C. scoticus (4)

    
 

[98.4]

[0]

[1.6]

  

Granges

C. obsoletus (88) {6}

C. grisescens II (1)

0

0

0

(500)

C. scoticus (9)

C. pulicaris (2)

   
 

[100]

[0]

[0]

  

Wädenswil

C. obsoletus (91) {5}

C. lupicaris (5) {1}

C. pallidicornis (1)

0

0

(620)

C. scoticus (2)

C. pulicaris (1)

   
 

[95]

[4.2]

[0.8]

  

Mühlethurnen

C. obsoletus(74) {4}

C. lupicaris (1)

0

C. segnis (1)

3

(670)

C. scoticus (19) {1}

    
 

C. dewulfi (2) {1}

    
 

[99.0]

[1.0]

[0]

  

Bennau

C. obsoletus (74) {4}

C. grisescens II (1) {1}

C. pallidicornis (1)

0

4

(810)

C. scoticus (11) {1}

C. lupicaris (9)

   
 

[92.1]

[5.6]

[2.2]

  

Château d’Oex

C. obsoletus (91) {6}

C. lupicaris (1)

0

C. newsteadi (1)

0

(940)

C. scoticus (3)

C. pulicaris (3)

   
  

C. punctatus (1)

   
 

[90.6]

[4.7]

[4.7]

  

Chaumont

C. obsoletus (12) {2}

C. lupicaris (1)

0

0

3

(1,110)

C. scoticus (75) {3}

C. pulicaris (1)

   
 

C. chiopterus (6)

C. punctatus (1)

   
 

C. dewulfi (1) {1}

    
 

[94.2]

[1.9]

[3.9]

  

Davos

C. obsoletus (5)

C. grisescens I (4) {1}

C. pallidicornis (1)

0

0

(1,560)

C. scoticus (2)

C. grisescens II (87) {5}

   
  

C. pulicaris (1)

   
 

[4.2]

[95.0]

[0.8]

  

Sils

C. obsoletus (4)

C. deltus (6) {1}

0

C. fascipennis (2)

0

(1,800)

C. scoticus (3)

C. grisescens I (5)

 

C. reconditus (1)

 
  

C. grisescens II (75) {5}

   
  

C. pulicaris (4)

   
 

[4.5]

[82.9]

[12.6]

  

Juf

 

C. grisescens I (51) {4}

0

C. fascipennis (3)

0

(2,130)

 

C. grisescens II (40) {2}

 

C. reconditus (2)

 
  

C. pulicaris (2)

 

C. segnis-like (2)6

 
 

[1.5]

[94]

[4.5]

  
  1. 1 Specimens randomly selected from a single catch from the high abundance season (August/September 2010), stored in 70% ethanol for more than 1 year and analysed using a database containing validated biomarker mass sets of 15 Culicoides species[27].
  2. 2 Meters above sea level.
  3. 3 PCR/sequencing[22] with 6 randomly chosen (http://www.random.org) specimens/site. Species identification by comparison with GenBank entries (>99% identity).
  4. 4 Percentage of Culicoides morphologically classified as belonging to Obsoletus group, Pulicaris group or other Culicoides of the catch from which the specimens were selected for the MALDI-TOF MS analyses.
  5. 4 Identified by PCR/sequencing[22].
  6. 5 No species identification achievable due to poor quality mass spectra.
  7. 6 Best match: 96% identity (403/422) with GenBank entry HQ824509 (Ccxx segnis from Switzerland).
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