Table 2 Repeat motif, primers sequence, and annealing temperature used for PCR amplification of the microsatellite loci in Phlebotomus papatasi collected from different populations in Sudan and Egypt

*Pap1

(GTT)

FAM-CGGTCACTTCCCTCTTCTCA

58°C

R: CCCCTTCTACAACCACTTCAA

*Pap2

(CA)

JOE-CACTCGCAAGATGGTAGGGTA

58°C

R: CGGCGCCTATAGACAGAGAA

*Pap4

(GAA)

FAM-GTGGCAAAATTGGTTTGGT

55°C

R: ACCTTTTGCATATGCCCATC

^♦ AA13

(GAG)

TAMRA-CTCCAACTCCTCATCCACCTC

59/55°C

R: GGCGACGAATGGGACAAAG

^♦ AA18

(AGC)

JOE-5’-GCTGCACAGCCGCCTGATG

55°C

R: ATCAGCAGACACTCCAGCAACACC

^♦ AA24

(GCA)

TAMRA-5’-CTATTCCCGCCCCACTTGG

55°C

R: TCAATCGACATTCGGACAGGC

^♦ AA82

(GGA)

JOE-TCAACCAAGGTAGTCTCATCAG

49°C

R: GAGGACTTCCGATTTCTGTAG