Table 2 Repeat motif, primers sequence, and annealing temperature used for PCR amplification of the microsatellite loci in Phlebotomus papatasi collected from different populations in Sudan and Egypt
| Locus | Motif | Fluorescent labeled primer sequence† | Annealing T° |
|---|---|---|---|
| *Pap1 | (GTT) | FAM-CGGTCACTTCCCTCTTCTCA | 58°C |
| R: CCCCTTCTACAACCACTTCAA | |||
| *Pap2 | (CA) | JOE-CACTCGCAAGATGGTAGGGTA | 58°C |
| R: CGGCGCCTATAGACAGAGAA | |||
| *Pap4 | (GAA) | FAM-GTGGCAAAATTGGTTTGGT | 55°C |
| R: ACCTTTTGCATATGCCCATC | |||
| ♦ AA13 | (GAG) | TAMRA-CTCCAACTCCTCATCCACCTC | 59/55°C |
| R: GGCGACGAATGGGACAAAG | |||
| ♦ AA18 | (AGC) | JOE-5’-GCTGCACAGCCGCCTGATG | 55°C |
| R: ATCAGCAGACACTCCAGCAACACC | |||
| ♦ AA24 | (GCA) | TAMRA-5’-CTATTCCCGCCCCACTTGG | 55°C |
| R: TCAATCGACATTCGGACAGGC | |||
| ♦ AA82 | (GGA) | JOE-TCAACCAAGGTAGTCTCATCAG | 49°C |
| R: GAGGACTTCCGATTTCTGTAG |
