Number of oocysts per midgut. Data were obtained from TPx-2 WT and two TPx-2 KO populations (KO1 and KO2). The line indicates the 95% confidence interval of the mean. The oocyst formation rate of the TPx-2 KO populations was the same as for the TPx-2 WT population (P > 0.05; Mann–Whitney test). The number of mosquitoes dissected was 36 for each experimental group. The figure shows representative results of three independent experiments.