Mouse-skin membrane-based semi-artificial feeding device. (A) Tick bite lesion of mouse skin used as a membrane in semi-artificial feeding technique. h, hypostome; ch, chelicera; ce, cement; en, endodermis; ep, epidermis; hy, hypodermis; fl, feeding lesion. (HE, Bar: 200 μm) (B) Lateral view of the semi-artificial feeding system with a feeding tick. (C) Schematic diagram of the system used in this study. (D-F) Chronological changes (0, 3, 5 h) of ticks feeding on the semi-artificial feeding system.