Phenotypic comparison of ticks fed on different feeding systems. (A) Engorged body weight of NEr (n = 6), AE (n = 4), and NEm (n = 6) ticks. The bars represent mean values and the error bars indicate the standard deviation. (B) Total egg mass weight measured at 10 DPO. (C) Egg conversion ratio (total egg weight/engorged body weight). (D) Comparison of hatchability of eggs (larval counts/total number of larvae and non-hatching eggs) derived from engorged ticks of different feeding systems. The results shown are from a single experiment and are representative of three independent experiments.