Table 1 Primers for quantitative RT-PCR
From: Semi-artificial mouse skin membrane feeding technique for adult tick, Haemaphysalis longicornis
Gene name and primer ID | Primer sequences (5′ to 3′) |
|---|---|
β-actin | |
HlActin8F1 | CCCATCTACGAGGGTTACGCTC |
HlActin9R1 | CATCTCCTGCTCGAAGTCCAGG |
HlSP (serine protease) | |
HlSPEf2 | TCCTTCCTCCTGAAGCAG |
HlSPEr2 | CGTTCGCTATCCATGGTC |
Longepsin (aspartic protease) | |
longepsin_f2 | CATGAACGGCGTGAAAGTAG |
longepsin_r2 | TCCTTGCCTTCCAAAACG |
Longipain (cysteine protease) | |
longipain_f1 | ACCCTGCGACAAGAGCATAC |
longipain_r1 | TCCACTTGAATCTGCGTCAC |
HlSCP1 (serine carboxypeptidase) | |
HlSCP1_f1 | TGCTTCAGACTGCATTGACC |
HlSCP1_r1 | TTGACCGCAGGTGTCATATC |
HlLgm (legumain) | |
HLleg1RTf1 | CGACGAGCAAATCGTAGTCA |
HLleg1RTr1 | ACTTTTCCGCTTCCTCCATT |
HlLgm2 (legumain) | |
B32G12f1 | CCTTCGCAACAAGCTAAAGG |
B32G12r1 | TCAGAAGTCCTTCGGTGCTT |
HlLAP (aminopeptidase) | |
HlLAPf1 | CGCTAAGAAGCAGGCTGTCCTA |
HlLAPr1 | TCAGACCGTAGAAAACTCTGGAC |
HlLAP2 (aminopeptidase) | |
HlLAP2f2 | AAGGCTCTTCACGAAGTGGA |
HlLAP2r1 | TGGTCGACACCTCGAACATA |
Hlgut-defensin (anti-microbial peptide) | |
EF132731 F | GGGACTTTTACTGGCTTTCCTG |
EF132731 R | ACACGCCCTTTCATCGAAC |