Distribution of CHIKV in 3DPI and 5DPI Ae. aegypti salivary glands. Salivary glands were dissected at 3 DPI (A) and 5 DPI (B). CHIKV labelled in red was identified using 3E4 anti-CHIKV according to the protocol described in the Methods section. Nuclei were labelled in blue using DAPI. Alexafluor 488-labelled phalloidin was used to label the actin network in green. A superimposition of all labelling is also shown.