Spots up-regulated at 5DPI in CHIKV-infected salivary glands of Ae. aegypti. 130 μg of salivary gland extract from 5 DPI CHIKV-infected mosquitoes and control mosquitoes were loaded onto 3–10 NL immobilins (18 cm). The immobilins were then deposited on the top of 12% SDS-PAGE gels. Spots were revealed using SYPRO Ruby. Gel profiles were compared using Image Master Platinum software. The spots that were found up-regulated at 5DPI are indicated by circles (fold change>1.8; Anova<0.05).