Skip to main content

Table 2 Diagnostic methods

From: A systematic review and meta-analysis of Toxoplasma gondii infection among the Mexican population

Diagnostic test Fundament Sensitivity Specificity Timeline References
Skin test antigen (STA Type IV cell-mediated hypersensitivity reaction against the T.gondii antigen. 80% 70% 1950-1951 [1619, 21, 49]
Sabin and Feldman Dye Test (SF). The gold standard. A dye test in which the serum antibodies alter the staining pattern of the T. gondii tachyzoites. 96% 98% 1955-2005 [20, 22, 23, 37, 44, 46]
Complement Fixation Test (CF). Antigen-antibody complexes are formed and detected by using a standard system with hemolisin and complement 97.1% 64.5% 1954-1982 [53]
Látex Flocculation Test (LF). This test uses latex particles for antigen-antibody flocculation. No reported. 1974 [35]
Indirect Fluorescent Antibody Test (IFI). Tachyzoites are fixed on a slide and exposed to test serum, then washed and exposed to a standard antibody labeled with fluorescent dye. 95% 96% 1986 to date [5, 25, 27, 28, 36, 38, 54]
Indirect Haemagglutination Test (IHA). This test uses sheep red cells exposed to tannic acid and then to the soluble antigen fixed at 37 °C. 95% 96% 1972 to 1989 [24, 26]
Enzyme-linked immunoabsorbent assay (ELISA). The ELISA detects T. gondii immunoglobulin IgG e IgM in serum and other body fluids with antibodies marked with peroxidase and fosfatase enzymes 100% 98.4% 1995 to date [6, 7, 2934, 3943, 47, 48, 5052, 55, 56]