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Figure 1 | Parasites & Vectors

Figure 1

From: Evaluation of protective immune responses induced by DNA vaccines encoding Toxoplasma gondii surface antigen 1 (SAG1) and 14-3-3 protein in BALB/c mice

Figure 1

Constructed plasmid maps and identification of the recombinant plasmids with restriction enzyme digestion. (A) The SAG1 gene was inserted into pBudCE4.1 plasmid by the Sal I and Xba I cloning sites. (B) The 14-3-3 gene was inserted with the Kpn I and Xho I sites. (C) The two genes were both inserted in pBudCE4.1 with appropriate cloning sites. (D) DNA Mark (lane M), pSAG1 digested with Sal I (lane 1), pSAG1 digested with Sal I/Xba I (lane 2), p14-3-3 digested with Kpn I (lane 3), p14-3-3 digested with Kpn I/Xho I (lane 4), pSAG1/14-3-3 digested with Sal I /Xba I (lane 5), pSAG1/14-3-3 digested with Kpn I/Xho I (lane 6).

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Parasites & Vectors

ISSN: 1756-3305

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