Figure 2From: Evaluation of protective immune responses induced by DNA vaccines encoding Toxoplasma gondii surface antigen 1 (SAG1) and 14-3-3 protein in BALB/c miceIn vitro expression analysis of the constructs in Hela cells by RT-PCR and Western blotting. (A) RT-PCR results for SAG1 and 14-3-3 (lane1 and lane 2) in cells transfected with pSAG1 or p14-3-3. (B) RT-PCR results for SAG1 and 14-3-3 (lane1 and lane 2) in cells transfected with pSAG1/14-3-3. (C) Pre-stained protein marker (lane M), hela cells transfected with pSAG1 (lane 1), cells transfected with p14-3-3 (lane 2), cells transfected with pSAG1/14-3-3 (lane 3).Back to article page