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Figure 1 | Parasites & Vectors

Figure 1

From: The use of Loop-mediated Isothermal Amplification (LAMP) to detect the re-emerging Human African Trypanosomiasis (HAT) in the Luangwa and Zambezi valleys

Figure 1

Visual appearance of representative results for repetitive insertion mobile element (RIME)-LAMP and human serum resistance-associated gene (SRA)-LAMP before and after treatment of a patient with Early (A) and Late (B) Stage human African trypanosomiasis (HAT). Loopamp Fluorescent detection reagent was added to the reaction mixture at the beginning of the assay. The reactions were incubated at 64°C for 30 minutes. In contrast to the light green background fluorescence in the negative samples, positive samples exhibit a bright fluorescent green colour when visualized under the transilluminator. NC: Negative control (distilled water); PC: Positive control (Trypanosoma brucei rhodesiense); BS: patient blood sample; CSF: patient cerebral spinal fluid sample.

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