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Table 1 2qPCR results from fecal samples animals other than dogs and cats

From: Duplex quantitative real-time PCR assay for the detection and discrimination of the eggs of Toxocara canis and Toxocara cati (Nematoda, Ascaridoidea) in soil and fecal samples

Animals Origins

N

Macro- micro-scopic observation

T. canis FAM Cq value

T. cati Cy5 Cq value

In-house qPCR Red610 Cq value

T. canis/ T. cati molecular identification

References

Calf

1

ND

> 40.00

> 40.00

36.81 ± 0.24

Negative

CAL016

Calves

7

ND

> 40.00

> 40.00

> 40.00

Negative

CAL140,176,107,112,19,26,79

Rabbit

1

ND

> 40.00

> 40.00

36.73 ± 0.52

Negative*

NEM003

Rabbits

2

ND

> 40.00

> 40.00

> 40.00

Negative

NEM009,014

Hen

1

ND

> 40.00

> 40.00

35.44 ± 0.49

Negative*

NEM013A

Hen

1

ND

> 40.00

> 40.00

35.81 ± 0.24

Negative*

NEM013B

Hen

1

ND

> 40.00

> 40.00

33.30 ± 1.54

Negative*

NEM023A

Hen

1

ND

> 40.00

> 40.00

33.88 ± 0.63

Negative*

NEM023B

Cow

1

ND

> 40.00

> 40.00

> 40.00

Negative

NEM015

Pigeon

1

ND

> 40.00

> 40.00

36.20 ± 1.09

Negative*

NEM016

Horses

2

ND

> 40.00

> 40.00

> 40.00

Negative

NEM024,025

Donkey

1

ND

> 40.00

> 40.00

> 40.00

Negative

NEM017

Pig

1

ND

> 40.00

> 40.00

> 40.00

Negative

NEM018

  1. * These samples displayed a positive signal with the in-house PCR, suggesting that they harbored other roundworms eggs.