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Table 3 2qPCR results from dog and cat fecal samples

From: Duplex quantitative real-time PCR assay for the detection and discrimination of the eggs of Toxocara canis and Toxocara cati (Nematoda, Ascaridoidea) in soil and fecal samples

Animals Origins

N

Macro- micro-scopic observation

T. canis FAM Cq value

T. cati Cy5 Cq value

In-house qPCR Red610 Cq value

T. canis/ T. cati molecular identification

References

Dogs

9

Negative

> 40.00

> 40.00

> 40.00

Negative

CN4,5,6,7,8,10,12,14,20

Dog

1

Negative

> 40.00

> 40.00

36.76 ± 0.08

Negative

CN1

Dog

1

Negative

> 40.00

> 40.00

35.86 ± 0.15

Negative

CN2

Dog

1

Negative

> 40.00

> 40.00

37.97 ± 0.15

Negative

CN9

Dog

1

Negative

> 40.00

> 40.00

36.27 ± 0.22

Negative

CN11

Dog

1

Negative

> 40.00

> 40.00

35.67 ± 0.92

Negative

CN13

Cats

7

Negative

> 40.00

> 40.00

> 40.00

Negative

CT1,3,4,5,6,7,8

Cat

1

Toxocara eggs and worms

> 40.00

34.68 ± 0.82

35.68 ± 0.24

Toxocara cati

CT2

Cat

1

Toxocara eggs

> 40.00

35.07 ± 0.55

34.00 ± 0.51

Toxocara cati

CT9

Cat

1

Toxocara eggs

> 40.00

36.28 ± 0.67

36.75 ± 0.20

Toxocara cati

CT10