Table 4 2qPCR and in -house qPCR assays on soil samples spiked with T. cati and/or A. suum eggs
Samples | Eggs origins | Amount of eggs Mean ± SD | T. canis Cq FAM Mean ± SD | T. cati Cq Cy5 Mean ± SD | In-house Cq Red610 Mean ± SD |
|---|---|---|---|---|---|
E25 | T. cati | 100 ± 30 | > 40.00 | 25.18 ± 0.06 | 25.83 ± 0.05 |
E26 | T. cati | 100 ± 30 | > 40.00 | 25.29 ± 0.02 | 25.97 ± 0.03 |
E27 | T. cati | 10 ± 3 | > 40.00 | 31.12 ± 1.03 | 32.00 ± 0.76 |
E28 | T. cati | 10 ± 3 | > 40.00 | 31.49 ± 0.53 | 29.53 ± 0.77 |
E29 | T. cati | 5 ± 2 | > 40.00 | 30.62 ± 0.84 | 31.49 ± 0.53 |
E30 | T. cati | 5 ± 2 | > 40.00 | > 40.00 | > 40.00 |
E31 | T. cati | 5 ± 2 | > 40.00 | 30.13 ± 1.01 | 31.10 ± 0.75 |
E9 | A. suum | 1020 ± 280 | > 40.00 | > 40.00 | 25.93 ± 0.06 |
E10 | A. suum | 102 ± 28 | > 40.00 | > 40.00 | 30.08 ± 0.22 |
E32 | A. suum | 102 ± 28 | > 40.00 | > 40.00 | 29.66 ± 0.15 |
E11 | A. suum | 10 ± 3 | > 40.00 | > 40.00 | 33.70 ± 1.47 |
E12 | A. suum | 5 ± 2 | > 40.00 | > 40.00 | > 40.00 |
E13 | No egg spiked | 0 | > 40.00 | > 40.00 | > 40.00 |