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Table 5 Molecular assays on actual soil samples collected from playgrounds and sandpits

From: Duplex quantitative real-time PCR assay for the detection and discrimination of the eggs of Toxocara canis and Toxocara cati (Nematoda, Ascaridoidea) in soil and fecal samples

Soil samples

T. canis Cq FAM Mean ± SD

T. cati Cq Cy5 Mean ± SD

In-house Cq Red610 Mean ± SD

E33

>40.00

>40.00

30.71 ± 1.44

E34

>40.00

>40.00

29.62 ± 0.03

E17

>40.00

>40.00

>40.00

E18

>40.00

>40.00

>40.00

E19

>40.00

>40.00

32.61 ± 0.40

E20

>40.00

>40.00

31.39 ± 0.25

E21

>40.00

>40.00

32.39 ± 0.37

E22

>40.00

>40.00

>40.00

E23

>40.00

>40.00

27.97 ± 0.16

E24

>40.00

>40.00

>40.00

  1. The samples E33, E34, E19, E20, E21 and E23 are negative for T. canis and T. cati eggs and positive for non-Toxocara Ascaridoidea, whereas samples E17, E18, E22 and E24 are negative for any Ascaridoidea.