2DE map of soluble proteins from the midgut of Ae. albopictus females. Proteins were separated in the first dimension across a non-linear pH range of 3–10 NL, and in the second dimension in a 12% SDS-PAGE. The proteins were detected after staining by colloidal Coomassie Brilliant Blue G-250. The protein spots identified by nLC-MS/MS (LTQ-Orbitrap) are numbered, and their identities are provided in Table 1. The numbers on the right side indicate the molecular mass standards expressed in kDa. In the left, optical micrography obtained with camera attached to stereoscope.