Table 1 Primers used in this study for the amplification of the 16S rRNA, groESL, gltA, dsb and gp36 genes from E. mineirensis (UFMG-EV) genomic DNA
Target | Primers* | Sequence | Expected size (Kb) |
|---|---|---|---|
16Sr RNA | 8 F9 | 5′- AGTTTGATCATGGCTCAG – 3′ | 1.4 |
1448R | 5′- CCATGGCGTGACGGGCAGTGTG – 3′ | ||
groEL | HS110 | 5′- TGGGCTGGTA(A/C)TGAAAT – 3′ | 1.4 |
HS6 | 5′- CCICCIGGIACIA(C/T)ACCTTC – 3′ | ||
gltA | gltAF1 | 5′- CTTCTGATAAGATTTGAAGTGTTTG – 3′ | 1.5 |
gltAR1 | 5′- CTTTACAGTACCTATGCATATCAATCC – 3′ | ||
dsb | dsbF2 | 5′- CTTAGTAATACTAGTGGCAAGTTTTCCAC – 3′ | 0.683 |
dsbR2 | 5′- GTTGATATATCAGCTGCACCACCG – 3′ | ||
gp36 | EC36-F113 | 5′- GTATGTTTCTTTTATATCATGGC – 3′ | 1.0 |
EC36-R1 | 5′- GGTTATATTTCAGTTATCAGAAG – 3′ |