New species of Ehrlichia isolated from Rhipicephalus (Boophilus) microplus shows an ortholog of the E. canis major immunogenic glycoprotein gp36 with a new sequence of tandem repeats

Table 6 Length and percent of nucleotide and aa homology of the 5’ end pre-repeat region between the orthologs of gp36 in Ehrlichia mineirensis (UFMG-EV) and related genes

		Nucleotide		aa
Source	Strain	Length¹	Homology²	Length³	Homology⁴
*Ehrlichia mineirensis*	(UFMG-EV)	422	-	141	-
*Ehrlichia canis gp36*	TWN1 [EF551366]	425	91.2	142	82
	Louisiana [DQ146151]	428	88.2	143	78
	Sao Paulo [DQ146154]	428	88.4	143	78
	Cameroon [DQ146155]	428	88.6	143	79
*Ehrlichia chaffeensis gp47*	Arkansas [DQ085430]	471	61.8	157	52
	Sapulpa [DQ085431]	461	62.1	154	53
	Jax [DQ146156]	461	60.7	154	51
	St Vincent [DQ146157]	461	62.1	154	53
*Ehrlichia ruminantium* mucin-like protein	Highway [AF308673]	410	54.9	137	38

1 - The length were determinate using the Tandem Repeats Finder database[30].
2 - Percent of nucleotide homology were calculated with MegAlign, DNAStar, USA. Comparing with E. mineirensis (UFMG-EV).
3 - The length was determined using ClustalW[20] in comparison with Ehrlichia mineirensis (UFMG-EV).
4 - Percent of aa homology were calculated with ClustalW[20]. Comparing with E. mineirensis (UFMG-EV).
Accession Numbers are from GenBank.

ISSN: 1756-3305